CD99 antibody

Catalog No. ABIN6251917

The Mouse Monoclonal anti-CD99 antibody has been validated for FACS, WB, IF and IHC (p). It is suitable to detect CD99 in samples from Human and Rat.

Quick Overview for CD99 antibody (ABIN6251917)

Target: CD99

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HO36-1-1

Product Details anti-CD99 Antibody

Purification: PEG precipitation

Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Isotype: IgM kappa

Application Details

Application Notes: Western blot: 1-2 μg/mL

Flow Cytometry: 5-10 μL/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the CD99 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background: CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]

Gene ID: 4267

UniProt: P14209