Keratin 10/13 antibody

Catalog No. ABIN6251937

This Mouse Monoclonal antibody specifically detects Keratin 10/13 in WB, FACS, IF and IHC (p). It exhibits reactivity toward Human and Cat.

Quick Overview for Keratin 10/13 antibody (ABIN6251937)

Target: Keratin 10/13

Reactivity: Human, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM262

Product Details

Purification: Protein G affinity chromatography

Immunogen: A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.25-0.5 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Cytokeratin 10 + 13 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10 + 13

Background: This antibody recognizes cytokeratin 10 (56.5  kDa) and cytokeratin 13 (53  kDa) in western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Gene ID: 3858, 3860

UniProt: P13645, P13646