KRT1 antibody

Catalog No. ABIN6251964

This Mouse Monoclonal antibody specifically detects KRT1 in IF, WB, FACS and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for KRT1 antibody (ABIN6251964)

Target: KRT1 (Cytokeratin HMW (KRT1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT1 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: 34BE12

Product Details anti-KRT1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10e6 cells

IF: 0.5-1.0 μg/mL

WB: 0.5-1.0 μg/mL

IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for KRT1

Target: KRT1 (Cytokeratin HMW (KRT1))

Alternative Name: HMW Cytokeratin

Background: This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.

Gene ID: 3848

UniProt: P04264