Desmoglein 1 antibody

Catalog No. ABIN6251967

This Mouse Monoclonal antibody specifically detects Desmoglein 1 in WB, IF, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for Desmoglein 1 antibody (ABIN6251967)

Target: Desmoglein 1 (DSG1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 18D4

Product Details anti-Desmoglein 1 Antibody

Purification: Protein G affinity chromatography

Immunogen: A recombinant protein corresponding to the intracellular domain of human Desmoglein 1 was used as the immunogen for the Desmoglein antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

Immunoprecipitation: suitable

Optimal dilution of the Desmoglein antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Desmoglein 1

Target: Desmoglein 1 (DSG1)

Alternative Name: Desmoglein 1

Background: Desmoglein 1 (DSG1) is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Gene ID: 1828

UniProt: Q02413

Pathways: Cell-Cell Junction Organization