TTF1 antibody

Catalog No. ABIN6252067

The Mouse Monoclonal anti-TTF1 antibody is suitable to detect TTF1 in samples from Human, Rat and Mouse. It has been validated for IF, IHC (p) and FACS.

Quick Overview for TTF1 antibody (ABIN6252067)

Target: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TTF1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: HBNK2-1

Product Details anti-TTF1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for the TTF1 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the TTF1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TTF1

Target: TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))

Alternative Name: TTF1

Background: Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.

Gene ID: 7080

UniProt: P43699

Pathways: Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour