MFGE8 antibody

Catalog No. ABIN6252079

The Mouse Monoclonal anti-MFGE8 antibody has been validated for WB, FACS, IF and IHC (p). It is suitable to detect MFGE8 in samples from Human and Dog.

Quick Overview for MFGE8 antibody (ABIN6252079)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFGE8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MFG-06

Product Details anti-MFGE8 Antibody

Specificity: Does not react with mouse and rat

Purification: Protein G affinity chromatography

Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the MFGE8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: MFGE8

Background: Recognizes a protein of 40-45  kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin, or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Gene ID: 4240

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interactome