dsDNA antibody

Catalog No. ABIN6252090

This Mouse Monoclonal antibody specifically detects dsDNA in FACS, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for dsDNA antibody (ABIN6252090)

Target: dsDNA (Double-Stranded DNA (dsDNA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This dsDNA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: SPM603

Product Details anti-dsDNA Antibody

Purification: Protein G affinity chromatography

Immunogen: Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.

Isotype: IgG3 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunocytochemistry (Acetone-fixed): 0.5-1 μg/mL for 30 min at RT

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for dsDNA

Target: dsDNA (Double-Stranded DNA (dsDNA))

Alternative Name: Double Stranded DNA

Target Type: Nucleotide

Background: Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.