WT1 antibody

Catalog No. ABIN6252155

Product Details anti-WT1 Antibody

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: A recombinant human protein was used as the immunogen for the WT1 antibody.

Clone: WLMT1-1

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the WT1 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 (WT1 Products)

Synonyms: AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody

Background: Wilms Tumor is a transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. [UniProt]

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation