HSPG2 antibody

Catalog No. ABIN6252164

This anti-HSPG2 antibody is a Rat Monoclonal antibody detecting HSPG2 in WB, IF, IHC (p) and FACS. Suitable for Human, Mouse, Cow, Fish, Monkey and Pig.

Quick Overview for HSPG2 antibody (ABIN6252164)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reactivity: Human, Mouse, Cow, Fish, Monkey, Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: A7L6

Product Details anti-HSPG2 Antibody

Purification: Protein G affinity chromatography

Immunogen: A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the Perlecan antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: Perlecan

Background: This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism