ANO1 antibody (AA 2-101)

Catalog No. ABIN6252317

The Mouse Monoclonal anti-ANO1 antibody (Clone DG1-1484) (ABIN6252317) specifically detects ANO1 in IF, FACS, WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ANO1 antibody (AA 2-101) (ABIN6252317)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DG1-1484

Product Details anti-ANO1 Antibody

Binding Specificity: AA 2-101

Purification: Protein G affinity chromatography

Immunogen: A recombinant human protein corresponding to amino acids 2-101 of human DOG1/ANO1 was used as the immunogen for this DOG 1 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG 1 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: DOG1

Background: DOG 1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]

Gene ID: 55107

UniProt: Q5XXA6