MLANA antibody
-
- Target See all MLANA Antibodies
- MLANA (Melan A (MLANA))
-
Reactivity
- Human, Mouse, Rat
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This MLANA antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- Purification
- Protein G affinity chromatography
- Immunogen
- Recombinant human Melan-A protein was used as the immunogen for this antibody.
- Clone
- M2-9E3
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product MLANA Primary Antibody
-
-
- Application Notes
-
ELISA: order BSA-free format for coating
Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IP: 0.5-1 μg/500 μg protein lysate
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
- Target
- MLANA (Melan A (MLANA))
- Alternative Name
- Melan-A (MLANA Products)
- Synonyms
- A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
- Background
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
- Gene ID
- 2315
- UniProt
- Q16655
-