EPX antibody

Catalog No. ABIN6252336

This Mouse Monoclonal antibody specifically detects EPX in IF, FACS and WB. It exhibits reactivity toward Human.

Quick Overview for EPX antibody (ABIN6252336)

Target: EPX (Eosinophil Peroxidase (EPX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPX antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Clone: EPO104

Product Details anti-EPX Antibody

Purification: Protein G affinity chromatography

Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome were used as the immunogen for this Eosinophil peroxidase antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Aliquot and Store at 2-8C. Avoid freez-thaw cycles.

Target Details for EPX

Target: EPX (Eosinophil Peroxidase (EPX))

Alternative Name: Eosinophil Peroxidase

Background: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).

Gene ID: 8288

UniProt: P11678