kappa Light Chain antibody

Catalog No. ABIN6252341

This anti-kappa Light Chain antibody is a Mouse Monoclonal antibody detecting kappa Light Chain in FACS, WB, IHC and IF. Suitable for Human.

Quick Overview for kappa Light Chain antibody (ABIN6252341)

Target: kappa Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This kappa Light Chain antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: L1C1

Product Details anti-kappa Light Chain Antibody

Specificity: Does not react with rat

Purification: Protein G affinity chromatography

Immunogen: Human B-Lymphoma Cells were used as the immunogen for this Kappa light chain antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-2.0 μg/million cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for kappa Light Chain

Target: kappa Light Chain

Background: This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Gene ID: 3514

UniProt: P01834, P01601