CD1b antibody

Catalog No. ABIN6252383

The Mouse Monoclonal anti-CD1b antibody (Clone RIV12) (ABIN6252383) specifically detects CD1b in FACS, IF and Func. The antibody is reactive with Human samples.

Quick Overview for CD1b antibody (ABIN6252383)

Target: CD1b (CD1B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD1b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: RIV12

Product Details anti-CD1b Antibody

Purification: Protein G affinity chromatography

Immunogen: Human peripheral lymphocytes were used as the immunogen for this CD1b antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Functional testing: order BSA/azide free format

IF: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD1b antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD1b

Target: CD1b (CD1B)

Alternative Name: CD1b

Background: This antibody recognizes CD1b, a 44  kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1b molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. It is also expressed in interdigitating cells.

Gene ID: 910

UniProt: P29016