TLR9 antibody (N-Term)

Catalog No. ABIN6252411

This Mouse Monoclonal antibody specifically detects TLR9 in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TLR9 antibody (N-Term) (ABIN6252411)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: TR9541

Product Details anti-TLR9 Antibody

Binding Specificity: N-Term

Purification: Protein G Affinity Chromatography

Immunogen: A recombinant protein fragment from the N-terminal portion of human TLR9 was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Western blot: 2-4 μg/mL

Flow Cytometry: 1 μg/10e6 cells

Immunohistochemistry (Paraffin): 3-5 μg/mL (1)

1. For optimal TLR9 staining, formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: TLR9

Background: The mammalian immune system is composed of two components: innate immunity and adaptive immunity. Toll-like receptors (TLRs) are an essential part of the innate immune system. Through recognition of pathogen-associated molecular patterns (PAMPs), TLRs mount an immune defense against pathogens. There are 10 mammalian TLRs (TLR1-10). Unmethylated CpG sequences in bacterial genomes and viral DNA molecules activates TLR9, leading to the inflammatory response.

Gene ID: 54106

UniProt: Q9NR96

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades