CD3 epsilon antibody

Catalog No. ABIN6252412

The Rabbit Polyclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, Chicken, Dog, Sheep, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Frog, Opossum and Primate. It has been validated for FACS, WB and IP.

Quick Overview for CD3 epsilon antibody (ABIN6252412)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, Chicken, Dog, Sheep, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Frog, Opossum, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CD3 epsilon Antibody

Purification: Protein A purified CD3e antibody

Immunogen: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry: use the CD3e antibody at 0.5-1 μg/million cells

WB: 0.5-1.0 μg/mL

IP: 1-2 μg/500 μg protein lysate



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway