CD3 epsilon antibody (AA 23-119)

Catalog No. ABIN6252413

The Mouse Monoclonal anti-CD3 epsilon antibody (Clone C3e-1308) (ABIN6252413) specifically detects CD3 epsilon in FACS, WB, IF and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD3 epsilon antibody (AA 23-119) (ABIN6252413)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: C3e-1308

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 23-119

Purification: Protein G affinity chromatography

Immunogen: Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Optimal dilution of the CD3e antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. It is required for normal immune responses. This antibody is specific to the epsilon chain of CD3.

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway