CD3 epsilon antibody (Cytoplasmic Domain)

Catalog No. ABIN6252415

The Mouse Monoclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Human, Mouse, Rat, Pig, Cow, Horse, Rabbit and Guinea Pig. It has been validated for FACS, WB, IF and IHC (p).

Quick Overview for CD3 epsilon antibody (Cytoplasmic Domain) (ABIN6252415)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PC3-188A

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 156-168, Cytoplasmic Domain

Purification: Protein G affinity chromatography

Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Isotype: IgG1

Application Details

Application Notes: WB: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Optimal dilution of the CD3e antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway