ECM1 antibody

Catalog No. ABIN6252532

The Mouse Monoclonal anti-ECM1 antibody has been validated for IHC, IF and FACS. It is suitable to detect ECM1 in samples from Human and Rat.

Quick Overview for ECM1 antibody (ABIN6252532)

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ECM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM217

Product Details anti-ECM1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ECM1

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Alternative Name: Extracellular Matrix Protein 1

Background: This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Gene ID: 1893

UniProt: Q16610