Nkx2-2 antibody

Catalog No. ABIN6252568

The Rabbit Polyclonal anti-Nkx2-2 antibody has been validated for IHC, IF and FACS. It is suitable to detect Nkx2-2 in samples from Human.

Quick Overview for Nkx2-2 antibody (ABIN6252568)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Nkx2-2 Antibody

Purification: Protein A affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for this NKX2.2 antibody.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10e6 cells in 0.1ml

IF: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 minutes at RT

Titering of the NKX2.2 antibody may be required for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

Gene ID: 4821

UniProt: O95096

Pathways: Dopaminergic Neurogenesis