ATG4B antibody (N-Term)

Catalog No. ABIN6252580

The Rabbit Polyclonal anti-ATG4B antibody has been validated for WB. It is suitable to detect ATG4B in samples from Human, Mouse and Rat.

Quick Overview for ATG4B antibody (N-Term) (ABIN6252580)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG4B Antibody

Binding Specificity: N-Term

Purification: Protein A affinity chromatography

Immunogen: A recombinant protein fragment from the N-terminal region of ATG4B was used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: Western blot: 4-6 μg/mL

Provided assay concentrations are suggestions only, ATG4B antibody titration may be required for optimal results.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background: Autophagy is the cellular mechanism of degradation and turnover of unnecessary or dysfunctional cellular components. The unneeded components are engulfed in vesicles called autophagosomes, where they are broken down and recycled. The 'autophagy-related gene' ATG4B is a regulator of autophagasome maturation. The microtubule-associated protein LC3-I is lipidated (called LC3-II in its lipidated state) and recruited to the autophagosomal membrane where it is of vital importance in the maturation of autophagosomes. ATG4B regulates LC3-II availability by modulating LC3-I lipidation.

Gene ID: 23192

UniProt: Q9Y4P1

Pathways: Autophagy