ARG antibody (AA 1-150)

Catalog No. ABIN6252585

This Mouse Monoclonal antibody specifically detects ARG in IF, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for ARG antibody (AA 1-150) (ABIN6252585)

Target: ARG (Arginase (ARG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARG antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: ARG1-1126

Product Details anti-ARG Antibody

Binding Specificity: AA 1-150

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase antibody.

Isotype: IgG3 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the Arginase antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ARG

Target: ARG (Arginase (ARG))

Alternative Name: Arginase

Background: This protein is involved in the pathway that synthesizes L-ornithine and urea from L-arginine.

Gene ID: 383

UniProt: P05089