Liver Arginase antibody (ARG1, C-Term)

Catalog No. ABIN6252586

The Mouse Monoclonal anti-Liver Arginase antibody has been validated for WB, IHC (p) and IF. It is suitable to detect Liver Arginase in samples from Human.

Quick Overview for Liver Arginase antibody (ARG1, C-Term) (ABIN6252586)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: T1ARG-1

Product Details anti-Liver Arginase Antibody

Binding Specificity: ARG1, C-Term

Purification: Protein G affinity chromatography

Immunogen: A C-terminal recombinant protein fragment from ARG1 was used as the immunogen for the Arginase 1 antibody.

Isotype: IgG3 kappa

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arginase 1

Background: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Gene ID: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin