Villin 1 antibody (AA 179-311)

Catalog No. ABIN6252737

This anti-Villin 1 antibody is a Mouse Monoclonal antibody detecting Villin 1 in WB, IF, FACS and IHC (p). Suitable for Human.

Quick Overview for Villin 1 antibody (AA 179-311) (ABIN6252737)

Target: Villin 1 (VIL1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: VIL1-1314

Product Details anti-Villin 1 Antibody

Binding Specificity: AA 179-311

Purification: Protein G affinity chromatography

Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT

Titration of the Villin antibody may be required for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin

Background: Recognizes a protein of ~93  kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Gene ID: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization