TIMP3 antibody (AA 175-211)

Catalog No. ABIN6252786

This Rabbit Polyclonal antibody specifically detects TIMP3 in WB, IHC, IP, FACS and IF. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog and Horse.

Quick Overview for TIMP3 antibody (AA 175-211) (ABIN6252786)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-TIMP3 Antibody

Binding Specificity: AA 175-211

Purification: Protein A affinity chromatography

Immunogen: Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry: use antibody at 0.5-1 μg/million cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IP: 1-2 μg/500 μg protein lysate

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP3

Background: TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

Gene ID: 7078

UniProt: P35625