FTL antibody (AA 38-165)

Catalog No. ABIN6252862

The Mouse Monoclonal anti-FTL antibody is suitable to detect FTL in samples from Human. It has been validated for WB, IHC, IF and FACS.

Quick Overview for FTL antibody (AA 38-165) (ABIN6252862)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FTL-1386

Product Details anti-FTL Antibody

Binding Specificity: AA 38-165

Purification: Protein G affinity chromatography

Immunogen: Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.

Isotype: IgG2b

Application Details

Application Notes: Western blot: 0.1-0.2 μg/mL

Flow Cytometry: 0.1-0.2 μg/10^6 cells

IF: 0.1-0.2 μg/mL

IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin Light Chain antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: FTL1

Background: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Gene ID: 2512

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis