ACTH antibody (AA 25-39)

Catalog No. ABIN6252886

The Mouse Monoclonal anti-ACTH antibody has been validated for IHC, IF, FACS and ELISA. It is suitable to detect ACTH in samples from Human.

Quick Overview for ACTH antibody (AA 25-39) (ABIN6252886)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTH antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

Clone: ADCT1-1

Product Details anti-ACTH Antibody

Binding Specificity: AA 25-39

Purification: Protein G affinity chromatography

Immunogen: Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone were used as the immunogen for this ACTH antibody.

Isotype: IgG1

Application Details

Application Notes: ELISA: order BSA free format for coating

Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

IHC (FFPE):0.5-1 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Citrate Buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Alternative Name: ACTH

Target Type: Hormone

Background: ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Gene ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity