Elastase 3B antibody (AA 82-238)

Catalog No. ABIN6252899

This anti-Elastase 3B antibody is a Mouse Monoclonal antibody detecting Elastase 3B in WB, ELISA, IF, FACS and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Elastase 3B antibody (AA 82-238) (ABIN6252899)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CELA3B-1257

Product Details anti-Elastase 3B Antibody

Binding Specificity: AA 82-238

Purification: Protein G

Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Isotype: IgG1

Application Details

Application Notes: ELISA: 2-4 μg/mL, order BSA free format for coating

Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

IHC-P: 1-2 μg/mL for 30 min at RT

Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: CELA3B

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Gene ID: 23436

UniProt: P08861