FMR1 antibody (pSer499)

Catalog No. ABIN6254033

This Rabbit Polyclonal antibody specifically detects FMR1 in WB, IHC and ELISA. It exhibits reactivity toward Rat. It has been mentioned in 4+ publications

Quick Overview for FMR1 antibody (pSer499) (ABIN6254033)

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-FMR1 Antibody

Binding Specificity: pSer499

Specificity: Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primates, sheep, Xenopus, zebra fish

Characteristics: Our FMRP (Ser499) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects FMRP (Ser499) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FMRP, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-FMR1 antibody (ABIN6254033)

Bartley, OKeefe, Blice-Baum, Mihailescu, Gong, Miyares, Karaca, Bordey: "Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP." in: eNeuro, Vol. 3, Issue 6, (2017) (PubMed).

Reynolds, Smith, Jefferson, Lugo: "Comparison of Equivalence between Two Commercially Available S499-Phosphorylated FMRP Antibodies in Mice." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143134, (2016) (PubMed).

Guimarães-Souza, Perche, Morgans, Duvoisin, Calaza: "Fragile X Mental Retardation Protein expression in the retina is regulated by light." in: Experimental eye research, Vol. 146, pp. 72-82, (2016) (PubMed).

Bartley, OKeefe, Bordey: "FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity." in: PLoS ONE, Vol. 9, Issue 5, pp. e96956, (2015) (PubMed).

Target Details for FMR1

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Alternative Name: FMR1

Background:

Synonyms: FMR 1 antibody, Fmr1 antibody, Fmr1 gene antibody, FMR1_HUMAN antibody, FMRP antibody, Fragile X mental retardation 1 antibody, Fragile X mental retardation 1 protein antibody, Fragile X mental retardation protein 1 antibody, Fragile X mental retardation protein antibody, fragile X mental retardation syndrome-related protein 1 antibody, fragile X mental retardation autosomal homolog 1 antibody, FRAXA antibody, fxr1 antibody, MGC87458 antibody, POF antibody, POF1 antibody, Protein FMR-1 antibody, Protein FMR1 antibody, wu:fb16f11 antibody, wu:fd18c10 antibody, zgc:66226 antibody

Description Logic: Our FMRP (Ser499) rabbit polyclonal phosphospecific primary antibody is produced . It detects FMRP (Ser499) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Gene Name Alternatives: FMR1

Molecular Weight: 71 kDa

Gene ID: 24948

UniProt: Q80WE1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development