MECP2 antibody (pSer421)

Catalog No. ABIN6254071

Cited in 1 publication. This anti-MECP2 antibody is a Rabbit Polyclonal antibody detecting MECP2 in WB and ELISA. Suitable for Mouse.

Quick Overview for MECP2 antibody (pSer421) (ABIN6254071)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MECP2 Antibody

Binding Specificity: pSer421

Specificity: Specific for endogenous levels of the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: human, non-human primate

Characteristics: Our MeCP2 (Ser421) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects MeCP2 (Ser421) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-MECP2 antibody (ABIN6254071)

Buck, ONeill, Stitzel et al.: "Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi ..." in: Epigenetics & chromatin, Vol. 13, Issue 1, pp. 13, (2020) (PubMed).

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MECP2

Background:

Synonyms: AUTSX 3 antibody, AUTSX3 antibody, DKFZp686A24160 antibody, Mbd 5 antibody, Mbd5 antibody, MECP 2 antibody, MeCP 2 protein antibody, MeCP-2 protein antibody, Mecp2 antibody, MECP2_HUMAN antibody, Methyl CpG binding protein 2 (Rett syndrome) antibody, Methyl CpG binding protein 2 antibody, Methyl-CpG-binding protein 2 antibody, MRX 16 antibody, MRX 79 antibody, MRX16 antibody, MRX79 antibody, MRXS 13 antibody, MRXS13 antibody, MRXSL antibody, PPMX antibody, RS antibody, RTS antibody, RTT antibody, WBP 10 antibody, WBP10 antibody

Description Logic: Our MeCP2 (Ser421) rabbit polyclonal phosphospecific primary antibody is produced . It detects MeCP2 (Ser421) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: MECP2

Molecular Weight: 55 kDa

Gene ID: 17257

UniProt: Q9Z2D6

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane