PRAS40 antibody (pThr356)

Catalog No. ABIN6254074

This anti-PRAS40 antibody is a Rabbit Polyclonal antibody detecting PRAS40 in WB and ELISA. Suitable for Drosophila melanogaster.

Quick Overview for PRAS40 antibody (pThr356) (ABIN6254074)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PRAS40 Antibody

Binding Specificity: pThr356

Specificity: Specific for endogenous levels of the ~60 kDa PRAS40 protein phosphorylated at Thr356. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Drosophila melanogaster

Characteristics: Our PRAS40 (Thr356) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects PRAS40 (Thr356) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 of Drosophila PRAS40, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1

Background:

Synonyms: 40 kDa proline rich AKT substrate antibody, 40 kDa proline-rich AKT substrate antibody, AKT1 S1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 antibody, AKT1S 1 antibody, AKT1S1 antibody, AKTS1_HUMAN antibody, Lobe antibody, MGC2865 antibody, PRAS 40 antibody, PRAS antibody, PRAS40 antibody, Proline rich akt substrate antibody, Proline rich Akt substrate 40 kDa antibody, Proline-rich AKT1 substrate 1 antibody

Description Logic: Our PRAS40 (Thr356) rabbit polyclonal phosphospecific primary antibody is produced . It detects PRAS40 (Thr356) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: AKT1S1

Molecular Weight: 60 kDa

UniProt: Q7JXA2

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect