Beclin 1 antibody (pSer234)

Catalog No. ABIN6254094

The Rabbit Polyclonal anti-Beclin 1 antibody has been validated for WB and ELISA. It is suitable to detect Beclin 1 in samples from Human. There is 1 publication available.

Quick Overview for Beclin 1 antibody (pSer234) (ABIN6254094)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Beclin 1 Antibody

Binding Specificity: pSer234

Specificity: Specific for endogenous levels of the ~60 kDa beclin1 protein phosphorylated at Ser234. Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: bovine, canine, mouse, non-human primate, rat, sheep

Characteristics: Our Beclin1 (Ser234) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Beclin1 (Ser234) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of human beclin-1, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: 1:250

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-Beclin 1 antibody (ABIN6254094)

Gassen, Hartmann, Zschocke, Stepan, Hafner, Zellner, Kirmeier, Kollmannsberger, Wagner, Dedic, Balsevich, Deussing, Kloiber, Lucae, Holsboer, Eder, Uhr, Ising, Schmidt, Rein: "Association of FKBP51 with Priming of Autophagy Pathways and Mediation of Antidepressant Treatment Response: Evidence in Cells, Mice, and Humans." in: PLoS medicine, Vol. 11, Issue 11, pp. e1001755, (2014) (PubMed).

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: BECN1

Background:

Synonyms: PG6 antibody, ATG 6 antibody, ATG6 antibody, ATG6 autophagy related 6 homolog antibody, Bcl-2-interacting protein beclin antibody, Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody, Beclin 1 autophagy related antibody, Beclin-1 antibody, Beclin1 antibody, BECN 1 antibody, Becn1 antibody, BECN1_HUMAN antibody, Coiled coil myosin like BCL2 interacting protein antibody, Coiled-coil myosin-like BCL2-interacting protein antibody, GT197 antibody, Protein GT197 antibody, VPS 30 antibody, VPS30 antibody

Description Logic: Our Beclin1 (Ser234) rabbit polyclonal phosphospecific primary antibody is produced . It detects Beclin1 (Ser234) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: BECN1

Molecular Weight: 60 kDa

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy