Clathrin Heavy Chain (CLTC) (N-Term) antibody

Catalog No. ABIN6254150

There is 1 publication for this product available. The Goat Polyclonal anti-Clathrin Heavy Chain antibody is suitable to detect Clathrin Heavy Chain in samples from Human, Mouse, Rat, Cow, Pig, Dog, Avian, Monkey, Rabbit, Chicken, Horse, Cat, Donkey, Goat, Guinea Pig, Hamster and Sheep. It has been validated for WB and IF.

Quick Overview for Clathrin Heavy Chain (CLTC) (N-Term) antibody (ABIN6254150)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Avian, Monkey, Rabbit, Chicken, Horse, Cat, Donkey, Goat, Guinea Pig, Hamster, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details

Binding Specificity: N-Term

Purpose: Anti-Clathrin HC

Sequence: MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV VII

Specificity: Detects endogenous levels of total CLTC by Western blot in the whole cell lysates (MDCK, At-T20, COS-7).

Characteristics: Clathrin, heavy chain (Hc)

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 50 aa to N-terminus of human CLTC produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:250-1:1,000, IF:1:25-1:250

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References

Caì, Postnikova, Bernbaum, Yú, Mazur, Deiuliis, Radoshitzky, Lackemeyer, McCluskey, Robinson, Haucke, Wahl-Jensen, Bailey, Lauck, Friedrich, OConnor, Goldberg, Jahrling, Kuhn: "Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway." in: Journal of virology, Vol. 89, Issue 1, pp. 844-56, (2015) (PubMed).

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin HC

Background:

Goat polyclonal to Clathrin Heavy Chain. Clathrin is formed by three clathrin heavy chains and three light chains. This protein is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in endocytosis of a variety of macromolecules and in the intracellular trafficking of receptors.

clathrin, clathrin heavy chain 1, clathrin heavy polypeptide (Hc), clathrin heavy polypeptide-like 2, CLTC, CHC17, CLH-17, CLTCL2, Hc, heavy chain (Hc) antibody.