ErbB2/Her2 antibody (C-Term)

Catalog No. ABIN6254225

The Goat Polyclonal anti-ErbB2/Her2 antibody is suitable to detect ErbB2/Her2 in samples from Human, Mouse, Rat, Dog, Monkey, Horse, Pig, Rabbit, Cow, Hamster, Avian, Cat, Chicken, Donkey, Goat, Guinea Pig and Sheep. It has been validated for WB, IHC (p) and IHC (fro).

Quick Overview for ErbB2/Her2 antibody (C-Term) (ABIN6254225)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse, Pig, Rabbit, Cow, Hamster, Avian, Cat, Chicken, Donkey, Goat, Guinea Pig, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: C-Term

Purpose: Anti-ERBB2

Sequence: MPHPPPAFSP AFDNLYYWDQ DPPERGAPPS TFKGTPTAEN PEYLGLDVPV

Specificity: Detects endogenous levels of ERBB2 by Western blot.

Characteristics: Erb-b2 receptor tyrosine kinase

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 1,177 aa to the C-terminus of human ERBB2 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2

Background:

Goat polyclonal antibody to ERBB2. This protein is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases containing a single transmembrane domain and has an approximate molecular weight of 185 kDa. ERBB2 contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signalling. It has been shown to be involved in embryonic development and cancer progression.

CD340, erb-b2 receptor tyrosine kinase 2, HER2, HER-2, HER-2/neu, MLN 19, NEU, NGL and TKR1 antibody.

Molecular Weight: 185 kDa

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development