TJP1 antibody (C-Term)

Catalog No. ABIN6254231

There are 2+ publications for this product available. The Goat Polyclonal anti-TJP1 antibody is suitable to detect TJP1 in samples from Human, Mouse, Rat, Pig, Cow, Dog, Guinea Pig, Rabbit, Horse, Monkey, Chicken, Avian, Cat, Donkey, Goat, Hamster and Sheep. It has been validated for WB, IF, IHC (p) and IHC (fro).

Quick Overview for TJP1 antibody (C-Term) (ABIN6254231)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Guinea Pig, Rabbit, Horse, Monkey, Chicken, Avian, Cat, Donkey, Goat, Hamster, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TJP1 Antibody

Binding Specificity: C-Term

Purpose: Anti-ZO1

Sequence: PQGAIPEGVE QEIYFKVCRD NSILPPLDKE KGETLLSPLV MCGPHGLKFL KPVELRLPHC DPKTWQNKCL PGDPNYLVGA NCVSVLIDHF

Specificity: Detects endogenous levels of total ZO1 by Western blot in whole cell and tissue lysates.

Characteristics: Tight junction protein 1

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:50-1:200, IHC-F:1:50-1:200

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-TJP1 antibody (ABIN6254231)

Kim, Yoon, Choi, Kim, Lim: "3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis." in: Frontiers in cell and developmental biology, Vol. 9, pp. 698292, (2021) (PubMed).

Pelkonen, Sato, Reinisalo, Kidron, Tachikawa, Watanabe, Uchida, Urtti, Terasaki: "LC-MS/MS Based Quantitation of ABC and SLC Transporter Proteins in Plasma Membranes of Cultured Primary Human Retinal Pigment Epithelium Cells and Immortalized ARPE19 Cell Line." in: Molecular pharmaceutics, Vol. 14, Issue 3, pp. 605-613, (2017) (PubMed).

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: ZO1

Background:

Goat polyclonal to TJP1 tight junction protein 1 (TJP1). TJP1 is located on a cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.

ZO-1 and zona occludens protein 1 antibody.

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization