PsbE antibody

Catalog No. ABIN6254845

Cited in 3+ publications. The Rabbit Polyclonal anti-PsbE antibody (ABIN6254845) specifically detects PsbE in WB. The antibody is reactive with Arabidopsis thaliana, Barley, Nicotiana tabacum, Oryza sativa, Spinach and Triticum aestivum samples.

Quick Overview for PsbE antibody (ABIN6254845)

Target: PsbE

Reactivity: Arabidopsis thaliana, Barley, Nicotiana tabacum, Oryza sativa, Spinach, Triticum aestivum

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-PsbE | Alfa subunit of Cytochrome b559 of PSII

Cross-Reactivity (Details): Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Spinacia oleracea, Triticum aestivum
Not reactive in: Chlamydomonas reinhardtii, diatoms, Synechococcus sp. PCC 7942

Predicted Reactivity: Cannabis sativa,Glycne max,Populus alba,Salvia miltiorrhiza,Solanum tuberosum

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide chosen from PsbE protein of Arabidopsis thaliana P56779

Application Details

Application Notes: 1 : 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Ido, Nield, Fukao, Nishimura, Sato, Ifuku: "Cross-linking evidence for multiple interactions of the PsbP and PsbQ proteins in a higher plant photosystem II supercomplex." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20150-7, (2014) (PubMed).

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Shang, Yan, Liu, Cao, Mei, Xin, Wu, Wang, Du, Jiang, Zhang, Zhao, Sun, Liu, Yu, Zhang: "The Mg-Chelatase H Subunit of Arabidopsis Antagonizes a Group of Transcription Repressors to Relieve ABA-Responsive Genes of Inhibition." in: The Plant cell, (2010) (PubMed).

Target Details

Target: PsbE

Background:

Background: Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, a and h subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The a and h subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage. Alternative protein name: PSII reaction center subunit V

Additional information: Cellular [compartment marker] of thylakoid membraneThis product can be sold containing ProClin if requested.

Molecular Weight: 9.25 kDa

Gene ID: 844745

UniProt: P56779