MITF antibody (pSer73, pSer180)

Catalog No. ABIN6255037

This anti-MITF antibody is a Rabbit Polyclonal antibody detecting MITF in WB, IHC, IF, ELISA and ICC. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for MITF antibody (pSer73, pSer180) (ABIN6255037)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-MITF Antibody

Binding Specificity: pSer73, pSer180

Specificity: Phospho-MITF (Ser180/73) Antibody detects endogenous levels of MITF only when phosphorylated at Serine 180/73

Cross-Reactivity: Human, Monkey, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human MITF around the phosphorylation site of Serine 180/73

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background:

Description: Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Gene: MITF

Molecular Weight: 52kDa

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding