SOX9 antibody (pSer181)

Catalog No. ABIN6255043

This anti-SOX9 antibody is a Rabbit Polyclonal antibody detecting SOX9 in WB, IHC, ELISA, IF and ICC. Suitable for Human, Mouse and Chicken.

Quick Overview for SOX9 antibody (pSer181) (ABIN6255043)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SOX9 Antibody

Binding Specificity: pSer181

Specificity: Phospho-SOX9 (Ser181) Antibody detects endogenous levels of SOX9 only when phosphorylated at Serine 181.

Predicted Reactivity: Pig,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human SOX9 around the phosphorylation site of Ser181.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background:

Description: Transcriptional regulator (PubMed:24038782). Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 (By similarity). Plays a role in chondrocyte differentiation (By similarity). Important for normal skeletal development (PubMed:24038782).

Gene: SOX9

Molecular Weight: 69kDa

Gene ID: 6662

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development