Cofilin antibody (pSer3)

Catalog No. ABIN6255180

The Rabbit Polyclonal anti-Cofilin antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect Cofilin in samples from Human, Mouse and Rat.

Quick Overview for Cofilin antibody (pSer3) (ABIN6255180)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Cofilin Antibody

Binding Specificity: pSer3

Specificity: Phospho-Cofilin (Ser3) Antibody detects endogenous levels of Cofilin only when phosphorylated at Serine 3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1

Background:

Description: Binds to F-actin and exhibits  pH -sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:11812157, PubMed:15580268, PubMed:21834987, PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

Gene: CFL1

Molecular Weight: 19kDa

Gene ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events