NRF2 antibody (pSer40)

Catalog No. ABIN6255303

The Rabbit Polyclonal anti-NRF2 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect NRF2 in samples from Human, Mouse and Rat.

Quick Overview for NRF2 antibody (pSer40) (ABIN6255303)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NRF2 Antibody

Binding Specificity: pSer40

Specificity: Phospho-Nrf2 (Ser40) Antibody detects endogenous levels of Nrf2 only when phosphorylated at S40.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Dog,Chicken

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human nrf2 around the phosphorylation site of Ser40.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: NFE2L2

Background:

Description: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Gene: NFE2L2

Molecular Weight: 90 kDa

Gene ID: 4780

UniProt: Q16236

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling