SMAD1 antibody (pSer465)

Catalog No. ABIN6255357

The Rabbit Polyclonal anti-SMAD1 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect SMAD1 in samples from Human, Mouse and Rat.

Quick Overview for SMAD1 antibody (pSer465) (ABIN6255357)

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SMAD1 Antibody

Binding Specificity: pSer465

Specificity: Phospho-Smad1 (Ser465) Antibody detects endogenous levels of Smad1 only when phosphorylated at Serine 465.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Smad1 around the phosphorylation site of Ser465.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1

Background:

Description: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Gene: SMAD1

Molecular Weight: 60kDa

Gene ID: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development