C-JUN antibody (pSer73)

Catalog No. ABIN6255488

This Rabbit Polyclonal antibody specifically detects C-JUN in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse, Rat and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for C-JUN antibody (pSer73) (ABIN6255488)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-C-JUN Antibody

Binding Specificity: pSer73

Specificity: Phospho-c-Jun (Ser73) Antibody detects endogenous levels of c-Jun only when phosphorylated at Serine 73

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human c-Jun around the phosphorylation site of Serine 73

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-C-JUN antibody (ABIN6255488)

Wu, Wang, Fang, Huang, Sun, Xiao, Yan: "MFAP5 promotes tumor progression and bone metastasis by regulating ERK/MMP signaling pathways in breast cancer." in: Biochemical and biophysical research communications, Vol. 498, Issue 3, pp. 495-501, (2018) (PubMed).

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: c-Jun

Background:

Description: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

Gene: JUN

Molecular Weight: 37kDa

Gene ID: 3725

UniProt: P05412, P17535

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins