Beclin 1 antibody (pSer93, pSer96)

Catalog No. ABIN6255566

This Rabbit Polyclonal antibody specifically detects Beclin 1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Beclin 1 antibody (pSer93, pSer96) (ABIN6255566)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Beclin 1 Antibody

Binding Specificity: pSer93, pSer96

Specificity: Phospho-Beclin 1 (Ser93/Ser96) Antibody detects endogenous levels of Beclin 1 only when phosphorylated at Ser93/96.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Beclin 1 around the phosphorylation site of Ser93/96.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: BECN1

Background:

Description: Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate, different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.

Gene: BECN1

Molecular Weight: 60kDa

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy