GATA4 antibody (pSer105)

Catalog No. ABIN6256094

This Rabbit Polyclonal antibody specifically detects GATA4 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for GATA4 antibody (pSer105) (ABIN6256094)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GATA4 Antibody

Binding Specificity: pSer105

Specificity: Phospho-GATA4 (Ser105) Antibody detects endogenous levels of GATA4 only when phosphorylated at Serine 105.

Predicted Reactivity: Pig,Bovine,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human GATA4 around the phosphorylation site of Ser105.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4

Background:

Description: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).

Gene: GATA4

Molecular Weight: 44kDa

Gene ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis