Cyclin B1 antibody (pSer147)

Catalog No. ABIN6256146

The Rabbit Polyclonal anti-Cyclin B1 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect Cyclin B1 in samples from Human, Mouse and Rat.

Quick Overview for Cyclin B1 antibody (pSer147) (ABIN6256146)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Cyclin B1 Antibody

Binding Specificity: pSer147

Specificity: Phospho-Cyclin B1 (Ser147) Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Serine 147.

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser147.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1

Background:

Description: Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Gene: CCNB1

Molecular Weight: 60kDa

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase