GATA4 antibody (pSer262)

Catalog No. ABIN6256227

The Rabbit Polyclonal anti-GATA4 antibody has been validated for WB, ELISA, IF, IHC and ICC. It is suitable to detect GATA4 in samples from Human, Rat and Mouse. There is 1 publication available.

Quick Overview for GATA4 antibody (pSer262) (ABIN6256227)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-GATA4 Antibody

Binding Specificity: pSer262

Specificity: Phospho-GATA4 (Ser262) Antibody detects endogenous levels of GATA4 only when phosphorylated at Serine 262.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human GATA4 around the phosphorylation site of Ser262.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-GATA4 antibody (ABIN6256227)

Karimi, Jalili, Mansouri, Bahremand, Gholami: "Effect of melatonin on steroidogenesis-related enzymes expression and testosterone synthesis following CoCl2-induced hypoxia in TM3 Leydig cells." in: Iranian journal of basic medical sciences, Vol. 26, Issue 9, pp. 1041-1046, (2023) (PubMed).

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4

Background:

Description: Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).

Gene: GATA4

Molecular Weight: 48kDa

Gene ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis