MKP-1/2 antibody (pSer296, pSer318)

Catalog No. ABIN6256252

Product Details anti-MKP-1/2 Antibody

Target: MKP-1/2 (DUSP1/4) (Dual Specificity Phosphatase 1/4 (DUSP1/4))

Binding Specificity: pSer296, pSer318

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKP-1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Phospho-MKP-1/2 (Ser296/318) Antibody detects endogenous levels of MKP-1/2 only when phosphorylated at Serine 296/318

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human MKP-1/2 around the phosphorylation site of Serine 296/318

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for MKP-1/2

Target: MKP-1/2 (DUSP1/4) (Dual Specificity Phosphatase 1/4 (DUSP1/4))

Alternative Name: MKP-1/2 (DUSP1/4 Products)

Background:

Description: Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

Gene: DUSP1

Molecular Weight: 45kDa

Gene ID: 1843

UniProt: P28562, Q13115