Synaptotagmin antibody (pSer309)

Catalog No. ABIN6256263

The Rabbit Polyclonal anti-Synaptotagmin antibody is suitable to detect Synaptotagmin in samples from Rat, Mouse and Human. It has been validated for WB, IHC, ELISA, IF and ICC.

Quick Overview for Synaptotagmin antibody (pSer309) (ABIN6256263)

Target: Synaptotagmin (SYT)

Reactivity: Rat, Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptotagmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Synaptotagmin Antibody

Binding Specificity: pSer309

Specificity: Phospho-Synaptotagmin (Ser309) Antibody detects endogenous levels of Synaptotagmin only when phosphorylated at Serine 309

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Serine 309

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for Synaptotagmin

Target: Synaptotagmin (SYT)

Alternative Name: Synaptotagmin

Background:

Description: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner, these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).

Gene: SYT1

Molecular Weight: 47kDa

Gene ID: 6857

UniProt: P21579, Q8N9I0