CENPA antibody (pSer7)

Catalog No. ABIN6256406

This Rabbit Polyclonal antibody specifically detects CENPA in WB and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for CENPA antibody (pSer7) (ABIN6256406)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CENPA Antibody

Binding Specificity: pSer7

Specificity: Phospho-CENP-A (Ser7) Antibody detects endogenous levels of CENP-A.

Cross-Reactivity: Human, Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CENP-A around the phosphorylation site of Ser7.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENP-A

Background:

Description: Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292).

Gene: CENPA

Molecular Weight: 17kDa

Gene ID: 1058

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location