TLK1 antibody (pSer743)

Catalog No. ABIN6256417

The Rabbit Polyclonal anti-TLK1 antibody (ABIN6256417) specifically detects TLK1 in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for TLK1 antibody (pSer743) (ABIN6256417)

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TLK1 Antibody

Binding Specificity: pSer743

Purpose: Phospho-TLK1 (Ser743) Antibody

Specificity: Phospho-TLK1 (Ser743) Antibody detects endogenous levels of TLK1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human TLK1 around the phosphorylation site of Ser743.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TLK1

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Alternative Name: TLK1

Background:

Description: Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

Gene: TLK1

Molecular Weight: 86kDa

Gene ID: 9874

UniProt: Q9UKI8